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Abstract Electrohydrodynamic (EHD) printing has been used in various applications (e.g., sensors, batteries, photonic crystals). Currently, research on studying the relationships between EHD jetting behaviors, material properties, and processing conditions is still challenging due to a large number of parameters, cost, time, and the complex nature of experiments. In this research, we investigated EHD printing behavior using a machine learning (ML)-guided approach to overcome limitations in the experiments. Specifically, we investigated two jetting modes and the size of printed material with a broader range of material properties and processing parameters. We used samples from both literature and our own experiment results with different type of materials. Different ML models have been developed and applied to the data. Our results have shown that ML can navigate a vast parameter search space to predict printing behavior with an accuracy of higher than 95% during EHD printing. Moreover, the results showed that ML models can be used to predict the printing behavior and feather size for new materials. The ML models can guide the investigation of EHD printing and helped us understand the printing behavior in a systematic manner with reduced time, cost, and required experiments.